Small Molecules Analysis

Creative Proteomics provides a mass spectrometry-based small molecule analysis service for researchers working in the fields of biology and pharmacology. This service provides qualitative and quantitative measurements of small molecules, revealing the complex structure of small molecules in their entirety and effectively advancing your research.

In chemistry, small molecules usually refer to biologically functional molecules with molecular weights less than 1000 Da (especially small molecules less than 400 Da). In biological, small molecules are biologically active small peptides, oligopeptides, oligosaccharides, oligonucleotides, etc. Given that most drugs are small-molecule drugs, small-molecule metabolites also play an important role, and the analysis of small molecules has received more and more attention. However, the content of small-molecule compounds in the sample is low, and other substances will cause greater interference to small molecules. The small molecule analysis service based on mass spectrometry technology can use isotope labeling to obtain accurate and reliable data, which can effectively solve the problem of insufficient sensitivity caused by complex biological matrices.

Service Content of Small Molecules Analysis

In the pharmaceutical industry, we use liquid chromatography-mass spectrometry (LC-MS) technology to provide customers with quantitative information on small molecule drugs to support PK/PD studies as well as toxicokinetic and bioequivalence data. In organic small molecule analysis, we utilize matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to detect small molecules with molecular weight < 500 Da, such as 2,5-dihydroxybenzoic acid (DHB), 9-Aminoacridine (9-AA), naphthylethylenediamine hydrochloride (NEDC), 1-naphthylhydrazine hydrochloride (NHHC), etc. We are also able to use high-resolution (HR) MS to determine the structure of small molecules.

Our main services are shown below.

Structural elucidation of small molecules.

Accurate mass analysis (mean MW) of small molecules.

Quantitative measurement of known small molecules in matrices from biological tissues.

Identification and quantitative measurement of small molecules in biological samples based on mass spectrometry combined with chromatographic and optical detection.

Mass Spectrometry Facilities and Projects

Bruker Impact II q-TOF

Bruker Ultraflextreme MALDI-TOF/TOF

Agilent 1200 HPLC with a Diode Array Detector (DAD)

Thermo Scientific™ Orbitrap Exploris™ 120 Mass Spectrometer

Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole-Orbitrap Mass Spectrometer

Requirements for Samples

• We focus on endogenous, microbial, and exogenous small molecules in a variety of samples including cell lysates, tissues, and biological fluids (e.g., plasma, urine).
• Please note that the sampling process for biological tissues needs to be performed at low temperatures. Store all samples at -80℃ and do not freeze and thaw repeatedly.
• Please ask our staff for specific sample requirements.

Deliverables

• Experimental steps
• Relevant mass spectrometry parameters
• Details of the identified phosphorylation sites
• Mass spectrometry images
• Raw data

Applications of Small Molecules Analysis

• Drug safety validation.
• Development of small molecule drugs.
• Identification of small molecule biomarkers for human diseases.
• Interpretation of metabolic pathways and understanding of disease mechanisms.

Advantages of Small Molecules Analysis

• Fully automated operation, effectively reducing human error.
• High throughput sample detection, greatly saving time and cost.
• High sensitivity, selectivity, and wide range of small molecule analysis.

Service Process

Creative Proteomics, a leading company in the mass spectrometry industry, can quickly help researchers identify and quantify small molecule compounds to probe the structures of small molecules and the networks of their interactions. If you are interested in our small molecule analysis service, please contact us today for a specific quote.