Mammalian Plasma Proteomes Quantitative Analysis

Creative Proteomics focuses on quantitative analysis services for proteomics. With our experienced team and advanced mass spectrometry facilities, we provide you with a recognized quantitative analysis service for mammalian plasma proteomes.

Mammalian plasma proteomes are a general term for proteins contained in mammalian plasma and are usually divided into three types: albumin, globulin, and fibrinogen. Plasma proteomes play an important physiological role and undertake various physiological functions such as maintaining osmotic pressure, transporting substances, and immune defense. Quantitative analysis of the mammalian plasma proteomes is of great significance. Because many diseases can lead to changes in the structure and quantity of proteins in plasma, such changes are of great significance for disease diagnosis and curative effect monitoring. The plasma proteomes quantitative analysis service provided by Creative Proteomics can help you explore the occurrence and development of diseases using liquid chromatography-mass spectrometry.

Service Content of Mammalian Plasma Proteomes Quantitative Analysis

In this service from Creative Proteomics, we deplete IgG and albumin in mammalian plasma for quantitative analysis. First, we processed the samples using multi-enzyme digestion filter aided sample preparation (MED FASP) and analyzed the samples using the Q-Exactive mass spectrometer. Then, protein abundance was calculated using the total protein method (TPA). The data were subsequently validated using stable isotope-labeled peptide-based protein quantification. At the same time, we were able to use the shotgun method for high-throughput, quantitative analysis of hundreds of plasma samples to quantify inflammatory markers such as the apolipoprotein family and C-reactive protein.

Mass Spectrometry Facilities and Projects

• Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer
• Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole-Orbitrap Mass Spectrometer

Requirements for Samples

• Each mammalian plasma sample should be greater than 1 µL and should be depleted of IgG and albumin.
• Please provide the specific concentration, volume, preparation time, and source of each sample. And inform the sample information as well as the control and experimental samples (if there is a group, describe the group information in detail.)

Deliverables

• Experimental steps
• Relevant mass spectrometry parameters
• Details of the identified phosphorylation sites
• Mass spectrometry images
• Raw data

Applications of Mammalian Plasma Proteomes Quantitative Analysis

• Search for differentially expressed proteins in diseases and plasma markers relevant for disease diagnosis.
• Analysis of proteins in plasma that are structurally and quantitatively altered is useful for diagnostic and efficacy monitoring of diseases.
• Mass spectrometry (MS)-based proteomics now allows for highly specific and quantitative readout of the plasma proteomes.

Service Process

Creative Proteomics provides a mammalian plasma proteomes quantitative analysis service based on an advanced mass spectrometry facility, and we are confident that we can provide you with a tailored protocol to help you perform high-throughput analysis of the plasma proteomes. If you are interested in our analytical service, please contact us today for support.

References

1. Bryk, A. H.; Wiśniewski, J. R. Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res . 2017, 16(8): 2752-2761.
2. Geyer, P. E.; et al . Plasma Proteome Profiling to Assess Human Health and Disease. Cell Syst . 2016, 2(3): 185-195.
3. Geyer, P. E.; et al . Plasma Proteome Profiling to detect and avoid sample-related biases in biomarker studies. EMBO Mol Med . 2019, 11(11): e10427.