With an experienced technical team and an advanced mass spectrometry platform, Creative Proteomics can provide professional and customized impact protein analysis services for research teams and scholars engaged in proteomics and related fields.
In proteomics studies, intact protein analysis of target proteins is performed using mass spectrometry for cleavage characterization, ligand binding, and modification analysis. This includes a variety of analyses such as determination of molecular weight size, peptide map analysis, and covalent interactions between proteins. Intact protein analysis provides a fingerprint of the sample and reveals protein differences between samples, such as post-translational modifications (PTM), etc. The Intact protein analysis service can provide researchers with useful protein table-related information in biotherapeutic and pharmaceutical research.
Service Content of Intact Protein Analysis
Creative Proteomics' mass spectrometry team can provide you with intact protein analysis services on enriched or purified proteins, targeting antibodies, protein couples, fusion proteins, oligonucleotides, and many other protein types. Proteins are analyzed in-depth and accurately by direct injection or by liquid chromatography (LC) combined with electrospray ionization (ESI) introduced into a mass spectrometer (MS). Most intact protein mixtures need to be separated before introduction into MS to reduce the complexity of precursor spectra and minimize ion suppression for highly accurate and sensitive experimental results.
Mass Spectrometry Facilities and Projects
- Electrospray Ionization Mass Spectrometry (ESI-MS)
- High Resolution and Accurate Mass Spectrometry (LC-MS)
- Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry (LC/Q-TOF)
Requirements for Samples
- Customers can provide dry powder or solution samples with recommended sample content of 10-50 µg protein and antibody sample concentration of not less than 90%.
- Please note that during sample preparation, do not use surfactants such as SDS, PEG, PPG, Tween, CHAPS, Triton, etc. Minimize the use of Tris, HEPES, PBS, MES, MOPS, etc.
- If your experimental samples must use surfactants and highly concentrated salt solutions in the preparation process, please remove the above reagents before sending the samples or inform us in advance, we will pre-treat the samples afterward for analytical services.
- Please provide detailed information for each sample, such as specific concentration, volume, preparation time, and source. (If there are groups, describe the group analysis information in detail.)
Our Advantages
- Competitive service prices in the industry with no hidden fees.
- We have a high-end mass spectrometry platform and advanced analytical instruments.
- We will strictly control the quality during the experiment and provide you with highly accurate experimental results.
- We can provide the optimal complete protein analysis solution for different samples, suitable for all kinds of protein samples.
Service Process
Creative Proteomics is a pioneer in the field of proteomics with advanced mass spectrometry facilities and a professional proteomics research team. We provide intact protein analysis service based on mass spectrometry technology. if you are interested in our intact protein analysis service, please contact us today for technical support.
The service is for research only, not for clinical use.
