Protein ADP-Ribosylation Identification

Creative Proteomics has rich experience in the identification of protein post-translational modifications. With a professional team and advanced mass spectrometry facilities, we can provide you with one-stop protein ADP ribosylation identification services from experimental design to result in delivery to accelerate your research progress.

ADP-ribosylation is a dynamic and reversible post-translational modification that refers to the process of covalently linking the ADP-ribose (ADPR) group to the target protease. ADPR is a modifier produced by NAD⁺ cleavage and can be added at one or more places on the protein, called mono or multi ADP-ribosylation. As one of the important post-translational modifications, ADP-ribosylation is involved in many important cellular processes, including cell signal transduction, DNA repair, gene regulation, and apoptosis. Inappropriate ADP-ribosylation has been implicated in certain forms of cancer. It is also the basis for bacterial compounds such as cholera, diphtheria, and other toxicities.

Service Content of Protein ADP-Ribosylation Identification

In the protein ADP-ribosylation identification service, Creative Proteomics uses advanced mass spectrometry facilities to identify ribosylated proteins, find the sites of ribosylation modification, and identify the type of ADP-ribosylation. In this service, we can provide analysis services including but not limited to Glu/Asp/Lys residues in mono-ADP-ribosylation, and analysis of multiple specific residues in poly-ADP-ribosylation. We need to first screen out the peptides of the target protein by primary mass spectrometry, and then collect all fragmented signals of the target peptide by secondary mass spectrometry. Then, the ADP ribosylation-modified proteins of interest were mainly quantitatively verified using LC-MS/MS.

Mass Spectrometry Facilities and Projects

• Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer
• Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer
• Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole-Orbitrap Mass Spectrometer
• Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS)

Requirements for Samples

• Solution (target protein): total target protein > 50 μg, target protein concentration > 80%.
• Solution (large-scale mixed protein): total protein >1 mg, protein concentration > 1 μg/μL.
• Please store all samples at -80°C, do not freeze and thaw samples repeatedly, and use sufficient dry ice for transportation.
• Please provide the specific concentration, volume, preparation time, and source of each sample. And inform the sample information as well as the control and experimental samples (if there is a group, describe the group information in detail.)

Deliverables

• Experimental steps
• Relevant mass spectrometry parameters
• Details of the identified phosphorylation sites
• Mass spectrometry images
• Raw data

Our Advantages

• One-stop service from experimental design to data analysis.
• Experienced working team and advanced mass spectrometry facilities.
• Strict adherence to international standards of laboratory and strict quality control requirements.

Service Process

Creative Proteomics has built a mature mass spectrometry platform, and our professional mass spectrometry identification team can quickly and accurately solve the problem of protein ADP-ribosylation identification for you. If you are interested in our protein ADP-ribosylation identification services, please contact us today for technical support.

References

1. Cohen, M. S.; Chang, P. Insights into the biogenesis, function, and regulation of ADP-ribosylation. Nat Chem Biol. 2018, 14(3): 236-243.