Creative Proteomics can provide comprehensive and systematic analysis and lipidome identification service of your samples, providing strong technical support for your lipidomics research.
Lipids are a class of essential biomolecules in the body, with a wide variety of properties, such as being soluble in non-polar organic solvents. Lipidome identification is mainly to study the composition and structure of lipids in samples through mass spectrometry facilities, to elucidate the metabolism of lipids and the mechanism of action in various life phenomena. Because lipids are involved in complex cellular physiological processes, abnormal lipid metabolism may lead to many diseases such as obesity, arteriosclerosis, and diabetes. With the deepening of researchers' understanding of the importance of lipid biological functions and the continuous innovation of mass spectrometry technology, lipidome identification based on mass spectrometry facilities has gradually become a research hotspot.
Service Content of Lipidome Identification
With the diversity and huge amount of lipids, the analysis of lipid molecular species and structures is very challenging. Creative Proteomics uses different lipid extraction methods to extract different species of lipids, such as fatty acids, glycerophospholipids, steroids, etc., or normal phase chromatography to separate lipids from biological samples at the species level based on the polarity differences of different lipid species. The lipids of biological samples are divided into different fractions such as phosphatidylcholines (GPChos), phosphatidylethanolamines (GPEtns), sphingolipids (SM), and cardiolipids (CL) according to the polarity of the lipid species. Then the lipid molecules (molecular species) in the fractions were further separated using reversed-phase chromatography, and then the lipid groups were identified using mass spectrometry. Finally, the assay data were analyzed in combination with several databases such as LIPID MAPS, Lipid Bank, and Cyber Lipids.
Mass Spectrometry Facilities and Projects
- LC-Triple Quad Mass Spectrometer
- Thermo Scientific™ HPLC-Q Exactive Orbitrap Mass Spectrometer
- Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer
- Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer
- Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole-Orbitrap Mass Spectrometer
Requirements for Samples
- Protein sample: 2-5 mg.
- The number of cell samples: 108.
- Animal tissue sample: 2 g, plant tissue: 400 mg.
- Blood/serum samples: 2 mL, urine samples: 10 mL
- The dry weight of yeast and microorganism samples: 400 mg.
- Please consult staff for other sample types.
- Please note that blood samples need to be anticoagulated with EDTA. And use plenty of dry ice when shipping samples.
Deliverables
- Experimental steps
- Relevant mass spectrometry parameters
- Details of the identified phosphorylation sites
- Mass spectrometry images
- Raw data
The Types of Lipids We Can Identify
Mainly includes cholesterol, cholesteryl esters and triglycerides, etc.
Mainly include glycerophospholipids, sphingolipids, and glycolipids, etc.
Mainly include long-chain acyl coenzyme A, long-chain acyl carnitine, fatty acids, ceramides, lysophospholipid molecules, arachidonic acid, diglycerides, sphingosine-3-phosphate, etc.
Applications of Lipidome Identification
- Drug Discovery
- Molecular Physiology
- Molecular Pathology
- Functional Genomics
- Nutrition and Environment and Health
Service Process
Creative Proteomics provides a one-stop lipidome identification service, effectively helping you uncover the relationship between lipid metabolism and the physiological and pathological processes of cells and even the body. If you are interested in our service, please contact us immediately for details.
The service is for research only, not for clinical use.
