Particular Post-Translational Modifications Quantitative Analysis

Creative Proteomics is a world-class protein quantitative analysis service provider. We have rich experience and mature technology and have been engaged in quantitative analysis of protein-specific post-translational modifications for a long time, which can effectively promote your research projects.

Protein post-translational modifications (PTMs) refer to chemical modifications of proteins during or after translation, which add different types of functional groups to proteins. Common protein post-translational modifications include phosphorylation, glycosylation, ubiquitination, and more. The quantitative analysis service of protein-specific post-translational modification can quantitatively analyze the specific modification of protein to help researchers explore the impact of the modification on protein-protein signal transduction and regulation, gene expression regulation, protein interaction, etc.

Service Content of Particular Post-Translational Modifications Quantitative Analysis

In the quantitative analysis of specific post-translational modifications, Creative Proteomics mainly uses MS and MS peptide mapping to identify specific protein post-translational modifications. In this service, we will provide pre-processing services, cut the protease into peptide fragments, and obtain relative molecular mass information by mass spectrometry. The amount of post-translational modification can be determined by a simple comparison of molecular weights compared to unmodified peptides. Quantitative analysis of post-translational modification sites can be achieved by secondary confirmation of the spectrum of secondary or multi-level mass spectrometry.

Mass Spectrometry Facilities and Projects

• Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer
• Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer
• Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole-Orbitrap Mass Spectrometer

Requirements for Samples

• We can accept animal tissue samples (including blood/urine/cerebrospinal fluid/lavage fluid/joint fluid), cell samples, microorganisms (bacteria/fungus), and other types of samples, and provide tissue samples pretreatment services.
• Please provide the specific concentration, volume, preparation time, and source of each sample. And inform the sample information as well as the control and experimental samples (if there is a group, describe the group information in detail.)

Deliverables

• Experimental steps
• Relevant mass spectrometry parameters
• Details of the identified phosphorylation sites
• Mass spectrometry images
• Raw data

Our Advantages

• Mass spectrometry facility with mass accuracy and high resolution.
• One-to-one project consulting services and customizable experimental protocols.
• Enable lab scalability with the TSQ Fortis mass spectrometer direct transfer method.

Service Process

Creative Proteomics ' high-quality quantitative analysis services can provide the most scientific solutions for researchers engaged in cell biology research and disease mechanism research. If you are interested in our particular post-translational modifications quantitative analysis service, please contact us today for a quote.