Creative Proteomics has been engaged in protein acetylation identification service for a long time and has a professional team and rich experience in mass spectrometry-based protein identification, which can provide efficient and accurate one-stop protein acetylation identification services for different project needs of customers.
Protein acetylation modification is the process of adding acetyl groups to protein lysine residues under the action of acetyltransferase. This process is a mechanism by which cells control gene expression, protein activity, or physiological processes. Acetylated proteins are mainly involved in a variety of cellular activities such as changing the structure of cellular chromosomes, activating transcriptional regulators in the nucleus, cell cycle, metabolism, and actin polymerization control.
Service Content of Protein Acetylation Identification
The protein acetylation identification service of Creative Proteomics takes complex samples such as tissues and cells as research objects and aims to identify acetylated proteins and corresponding acetylation sites in the samples. We can provide a one-stop protein acetylation identification service including protein extraction, protease digestion, acetylated peptide enrichment, peptide separation, mass spectrometry analysis and identification, and bioinformatics analysis.
Mass Spectrometry Facilities and Projects
- Sciex Triple TOF 6600 Mass Spectrometer
- Thermo Fisher Q Exactive HF Mass Spectrometry Platform
- Thermo Fisher Orbitrap Fusion Mass Spectrometry Platform
- Thermo Fisher Orbitrap Fusion Lumos Mass Spectrometry Platform
Requirements for Samples
- For cell samples, the total number of cells should be greater than 1 × 107 .
- For microbial samples, the dry weight should be greater than 200 mg.
- For protein samples, the total amount should be greater than 100 μg.
- For solid samples, animal tissue samples should be greater than 1 g, and plant tissue samples should be greater than 200 mg.
- Please note that for plasma samples use EDTA solution to prevent coagulation. And store all samples at -80°C, do not freeze and thaw samples repeatedly, and use sufficient dry ice for transportation.
- Please provide the specific concentration, volume, preparation time, and source of each sample. And inform the sample information as well as the control and experimental samples (if there is a group, describe the group information in detail.)
Deliverables
- Experimental steps
- Relevant mass spectrometry parameters
- Details of the identified phosphorylation sites
- Mass spectrometry images
- Raw data
Our Advantages
- Cost-effective and no hidden charges.
- We can customize your personalized program for different project needs.
- Professional protein acetylation identification team with service process conforming to international standards.
Service Process
Creative Proteomics is a professional company in the field of protein modification identification, which can provide customers with high-quality protein acetylation identification services. If you are interested in our protein acetylation identification service, please contact us today for technical support.
The service is for research only, not for clinical use.
