De Novo Peptides Sequencing

Creative Proteomics has rich experience in peptide de novo sequencing. We have built a complete and advanced technology platform to provide you with fast and accurate sequencing results to meet any of your research needs.

Both peptides and proteins are the basic building blocks of the human body and are substances made up of amino acids and held together by peptide bonds. There are thousands of peptides in total, and they are involved in complex physiological processes of life, such as hormone regulation, nerve impulse transduction, growth promotion, and anti-infection. In recent years, the advantages of polypeptide drugs in disease treatment have also been found, with high selectivity and high efficacy, and relatively safe and well-tolerated. Targeted de novo sequencing of peptides, the discovery of new peptides, or identification of peptide structures can effectively promote the progress of peptide drugs in drug research and development.

Service Content of De Novo Peptides Sequencing

In this service, we have different ways of sample processing. For relatively short peptides, Creative Proteomics uses secondary fragmentation to obtain mass spectrometry raw data, and de novo sequencing to determine the theoretical sequence of the peptide. For long peptides with more than 30 amino acids, we will use different proteases to digest and identify the target peptides respectively. After obtaining fragmented peptide fragments, the peptide sequence will be compared and spliced, and then de novo determined by sequencing.

Mass Spectrometry Facilities and Projects

• Nano HPLC-7T SolariX XR FTICR-MS
• Nano HPLC-Orbitrap Fusion™ Lumos™ Tribrid™ MS
• Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer

Requirements for Samples

• We accept protein samples over 20 µg, both in solution and lyophilized powder. If it is a solution sample, the buffer does not contain detergents such as high salt and SDS.
• Please provide the specific concentration, volume, preparation time, and source of each sample. And inform the sample information as well as the control and experimental samples (if there is a group, describe the group information in detail.)

Deliverables

• Experimental steps
• Relevant mass spectrometry parameters
• Details of the identified phosphorylation sites
• Mass spectrometry images
• Raw data

Our Advantages

• Competitive service prices to meet different project needs of customers.
• Strict quality control throughout the process, fully compliant with relevant laws and regulations.
• Advanced laboratory facilities and a professional technical team can provide fast and accurate experimental results.

Service Process

Creative Proteomics is the world's leading protein sequencing company. With its advanced mass spectrometry platform, we can provide you with high-quality peptide de novo sequencing service and guarantee to solve any problems you encounter. If you are interested in our service, please contact us today for technical support.