Creative Proteomics has rich experience in di-methyl labeling proteomics analysis. With a professional team and advanced mass spectrometry facilities, we can provide you with a one-stop di-methyl labeling proteomics analysis service from experimental design to result in delivery, to accelerate your research progress.
Di-methyl labeling technology mainly uses small molecule isotope chemical labels to label the N-terminus of proteins or polypeptides after cell lysis or the ε amino group of lysine by chemical labeling, so that proteins or polypeptides are labeled with different di-methyl labels. The di-methyl labeling technique is also well compatible with mass spectrometry, with high analytical precision and analytical breadth. Although di-methyl labeling will be affected by other substances in the labeling system, di-methyl labeling technology is still a high-quality and inexpensive labeling method and is widely used by researchers in proteomic analysis.
Service Content of Di-Methyl Labeling Proteomics Analysis
Di-methyl stable isotope labeling has developed into an important method for quantitative proteomics research due to its high labeling efficiency, accurate quantification, simple operation, and wide application range. In the di-methyl labeling proteomics analysis service, Creative Proteomics uses a mixture of reagents (i.e., unlabeled and stable isotope-labeled forms of cyanoborohydride and formaldehyde) to label primary amines (i.e., lysine's) in proteins or peptides N-terminal and ε-amino groups), followed by rapid and straightforward 2-plex or 3-plex quantitative proteomic analysis of various types of samples (eg, lysates, tissues) via mass spectrometry facilities.
Mass Spectrometry Facilities and Projects
- Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer
- Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer
- Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole-Orbitrap Mass Spectrometer
Requirements for Samples
We can accept almost all types of samples.
- Solution (target protein): total target protein > 50 μg, target protein concentration > 80%.
- Solution (large-scale mixed protein): total protein > 1 mg, protein concentration > 1 μg/μL.
- Please note that all samples should be stored at -80°C, do not freeze and thaw samples repeatedly, and use sufficient dry ice for transportation to reduce the possibility of sample degradation during transportation.
- Please provide the specific concentration, volume, preparation time, and source of each sample. And inform the sample information as well as the control and experimental samples (if there is a group, describe the group information in detail.)
Deliverables
- Experimental steps
- Relevant mass spectrometry parameters
- Details of the identified phosphorylation sites
- Mass spectrometry images
- Raw data
Our Advantages
- This analytical method applies to almost any sample.
- Stable isotope di-methyl labeling uses inexpensive reagents.
- A variety of alternatives can be provided to fully guarantee the accuracy of the analytical data.
- From experimental consultation to issuing analysis reports, we provide one-stop full-process technical support.
Service Process
Creative Proteomics has unique insights into the proteomic analysis. We can accurately provide you with a di-methyl labeling proteomic analysis service to solve any problems you encounter in scientific research projects. If you are interested in our di-methyl labeling proteomic analysis service, please contact us today for a detailed protocol.
The service is for research only, not for clinical use.
