Creative Proteomics is an expert in C-terminal sequence analysis. We provide one-to-one consulting services to customers around the world and develop customized mass spectrometry-based C-terminal sequencing solutions according to customer needs. We are confident to meet all your experimental needs.
The C-terminal sequence is an important structural and functional site of proteins and polypeptides and plays a decisive role in the biological functions of proteins. In addition, C-terminal cleavage is one of the important post-translational modifications of proteins. With the deepening of proteomic research, protein C-terminal sequencing will play an increasingly important role in the study of its function. There are three kinds of protein C-terminal sequencing: carboxypeptidase method, chemical method, and tandem mass spectrometry method. The continuous establishment of new methods for C-terminal sequencing will play a greater role in proteome research. Creative Proteomics focuses on providing customers with a mass spectrometry-based C-terminal sequence analysis service.
Service Content of C-Terminal Sequence Analysis
In the C-terminal sequence analysis service, we provide efficient and accurate C-terminal sequencing services based on advanced ESI-TOF and MALDI TOF. First, we used multiple enzymes to obtain redundant peptides that exhaustively define the C-terminal region of the purified protein. Combined with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), the C-terminal sequence analysis of these peptides can detect the blocked and modified protein C-terminal, confirm whether the recombinant protein is fully expressed, and detect whether the recombinant protein expression process occurs break and whether the C-terminal sequence of the recombinant protein is modified, etc.
Mass Spectrometry Facilities and Projects
- ESI-TOF Mass Spectrometer
- Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS)
Requirements for Samples
- Protein samples can be dry powders, solutions, or fixed on PVDF membranes and stained with Ponceau.
- Sample concentration and purity: concentration > 50 pmol, purity > 90% (recommended sample volume is more than 5 times the required amount).
- Salt content: non-volatile inorganic salts < 5 mM, volatile inorganic salts < 20 mM, Tris buffer system cannot be used.
- Please provide the specific concentration, volume, preparation time, and source of each sample. And inform the sample information as well as the control and experimental samples (if there is a group, describe the group information in detail.)
Deliverables
- Experimental steps
- Relevant mass spectrometry parameters
- Details of the identified phosphorylation sites
- Mass spectrometry images
- Raw data
Our Advantages
- High sensitivity and reproducibility, enabling application at the proteomic level
- A short experimental cycle time allows for a comprehensive bioinformatic analysis service.
- Our C-terminal sequence analysis method can be applied to large proteins after fragmentation with specific enzymes.
Service Process
As a professional protein sequence analysis company, Creative Proteomics is looking forward to providing you with the recognized C-terminal sequence analysis service. If you are interested in our C-terminal sequence analysis service, please contact us directly to communicate with the experts in detail.
The service is for research only, not for clinical use.
