Qualitative Analysis of Metabolites

Creative Proteomics has been committed to metabolomics-related research for a long time and has accumulated rich experience from multiple projects. It can provide the most authoritative metabolite qualitative analysis services to help you solve any problems in qualitative metabolomics research.

Metabolites, as the basis of organism phenotype, can quickly reveal the essence of life activities. Qualitative metabolomics analysis can qualitatively analyze all endogenous small molecule metabolites, usually with higher concentrations, and obtain more complete and rich metabolite information. It is easier to get a comprehensive metabolic profile as a whole, thus avoiding the misdirection of metabolomics studies. Qualitative metabolomics analysis can find out the different metabolites in the sample, and through bioinformatics analysis, find out the metabolic pathway, provide a comprehensive research perspective for metabolomics research. And can be used for marker screening, molecular mechanism research, and more integrative omics analysis, etc.

Service Content of Qualitative Analysis of Metabolites

Creative Proteomics will collect as much information as possible from a given sample. We first process the sample by applying general sample preparation techniques, and chromatographic separation techniques for analytes with different chemical groups. Metabolites are then detected using high-resolution mass spectrometry facilities. Finally, we apply a variety of bioinformatics software to analyze the resulting data, annotate metabolites, and provide you with the most accurate mass spectrometry reports.

Mass Spectrometry Facilities and Projects

• Thermo Scientific™ UHPLC-Q Exactive Orbitrap Mass Spectrometer
• Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer
• Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer
• Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole-Orbitrap Mass Spectrometer

Requirements for Samples

• In addition to common cells, tissues, and blood, metabolomics analysis can also be performed on feces, body fluids, exosomes, and cell culture fluids.
• Blood samples should be larger than 0.2 mL, please add blood anticoagulant.
• For tissue samples, the sample should be greater than 200 mg.
• For protein samples, please ensure that the total amount of protein is greater than 20 µg, acceptable solutions, and lyophilized powders.
• For cell samples, the number of cells should be greater than 10⁷.
• Immediately after sample collection, cryopreserve samples to quench any biological and/or abiotic chemical reactions. At the same time, customers are requested to provide as many biological sample replicates as possible.
• Please provide the specific concentration, volume, preparation time, and source of each sample. And inform the sample information as well as the control and experimental samples (if there is a group, describe the group information in detail.)

Deliverables

• Experimental steps
• Relevant mass spectrometry parameters
• Details of the identified phosphorylation sites
• Mass spectrometry images
• Raw data

Types of Data Analysis We Offer

• PCA statistical analysis. Knowing the ratio of different metabolites in different samples is extremely important and valuable for the screening of differential metabolites, especially for the screening of important molecular markers.
• PLSA-DA statistical analysis. Capable of extracting the maximum amount of simplified information from complex data for analysis.
• Metabolite functional analysis. Analyze the mechanism of metabolites and protein/gene interactions through statistical analysis such as PCA and clustering, and gain insight, into the function and role of metabolites in the organism.

Service Process

Creative Proteomics has provided high-quality metabolomics analysis services to a large number of clients. With our professional mass spectrometry facilities and scientific team, we can bring you a different service experience. If you are interested in our qualitative analysis service of metabolites, please contact us today for expert support.