Protein Glycosylation Identification

Creative Proteomics has extensive experience in protein glycosylation identification. With our professional team and advanced mass spectrometry facilities, we can provide you with a one-stop protein glycosylation identification service from experiment design to data analysis to meet any of your requirements.

Protein glycosylation is the process of linking carbohydrates in proteins under the action of glycosyltransferases. Amino acid residues on carbohydrates and proteins form glycosidic bonds, and various functional types of sugar chains are formed under the cleavage and processing of various enzymes. According to their sugar chain structure and glycosylation sites, they can be divided into two categories: N-glycoproteins and O-glycoproteins. Glycosylation modification not only affects the spatial conformation, biological activity, transport, and localization of proteins, but also plays a crucial role in specific biological processes such as molecular recognition, cell communication, and signal transduction.

Service Content of Protein Glycosylation Identification

In this service, Creative Proteomics uses mass spectrometry to identify glycoproteins, find the sites of glycosylation modification, and analyze the structure of sugar chains, to help researchers reveal the roles and functions of glycoproteins in life activities and biological function. Our service includes but is not limited to N-glycan analysis and identification service, O-glycan analysis and identification service, carbohydrate analysis, and identification service. We provide a one-stop protein glycosylation modification identification service from protein extraction, proteolytic digestion, glycosylation peptide enrichment, peptide separation, mass spectrometry identification, and data analysis.

Mass Spectrometry Facilities and Projects

• Sciex Triple TOF 6600 Mass Spectrometer
• Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer
• Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer
• Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole-Orbitrap Mass Spectrometer
• Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS)

Requirements for Samples

• The total number of cells > 1 × 10⁷.
• Microbial sample dry weight > 200 mg.
• The total amount of protein sample is more than 100 μg.
• Animal tissue samples > 1 g, plant tissue samples > 200 mg.
• Blood (plasma) samples > 1 mL, serum samples > 0.2 mL, and urine samples > 2 mL.
• Please note that for plasma samples use EDTA solution to prevent coagulation. And store all samples at -80°C, do not freeze and thaw samples repeatedly, and use sufficient dry ice for transportation.
• Please provide the specific concentration, volume, preparation time, and source of each sample. And inform the sample information as well as the control and experimental samples (if there is a group, describe the group information in detail.)

Deliverables

• Experimental steps
• Relevant mass spectrometry parameters
• Details of the identified phosphorylation sites
• Mass spectrometry images
• Raw data

Our Advantages

• Affordable service to meet different project needs of customers.
• High standard laboratory requirements and internationally recognized laboratory reagents.
• The experienced protein glycosylation identification team ensures the authenticity and credibility of the data.

Service Process

Creative Proteomics has built a mature mass spectrometry facility platform, and we can quickly and accurately solve any problems in protein glycosylation identification for you. If you are interested in our protein glycosylation identification services, please contact us today for technical support.