Creative Proteomics is able to provide mass spectrometry-based protein ubiquitination services for researchers with our advanced mass spectrometry platform. We can provide a one-stop protein ubiquitination service to meet different experimental needs.
The addition of ubiquitin to a substrate protein is called ubiquitination of the protein. Protein ubiquitination is a dynamic, multifaceted post-translational modification implicated in nearly all aspects of eukaryotic biology. Ubiquitination involves three major steps: activation, binding, and ligation, which are performed by ubiquitin-activating enzymes (E1), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s), respectively. Protein ubiquitination is an important post-translational modification of proteins. The ubiquitin-proteasome system mediates 80%-85% of protein degradation in eukaryotes. The ubiquitination of proteins runs through all stages of the cell life cycle, and by regulating the stability and activity of proteins, it is widely involved in DNA damage repair, gene transcription, apoptosis, signal transduction, and protein translation.
Service Content of Protein Ubiquitination Identification
Although post-translational ubiquitination-modified proteins are low in content and have a wide dynamic range in biological samples, Creative Proteomics has unique insights into protein enrichment, which can effectively improve protein abundance. The obtained ubiquitinated peptide samples were collected for analysis and identification. In this service, we can provide one-stop protein ubiquitination identification services from protein extraction, proteolytic digestion, ubiquitinated peptide enrichment, peptide separation, mass spectrometry identification, data analysis, etc.
Mass Spectrometry Facilities and Projects
- Sciex Triple TOF 6600 Mass Spectrometer
- Thermo Fisher Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer
- Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole-Orbitrap™ Mass Spectrometer
Requirements for Samples
- For cell samples, the sample cell number is > 1 × 108.
- For microbial samples, the microbial sample dry weight is > 400 mg.
- For protein samples, please ensure that the total amount of protein is 2-5 mg.
- For tissue solid samples, animal tissue samples > 2 g, plant tissue samples > 400 mg.
- For tissue fluid samples, blood (plasma) samples should be > 2 mL, serum samples should be > 2 mL, and urine samples should be > 10 mL.
- Please note that for plasma samples use EDTA solution to prevent coagulation. And store all samples at -80°C, do not freeze and thaw samples repeatedly, and use sufficient dry ice for transportation.
- Please provide the specific concentration, volume, preparation time, and source of each sample. And inform the sample information as well as the control and experimental samples (if there is a group, describe the group information in detail.)
Deliverables
- Experimental steps
- Relevant mass spectrometry parameters
- Details of the identified phosphorylation sites
- Mass spectrometry images
- Raw data
Our Advantages
- Fast experimentation cycle and competitive service price.
- Experienced and professional protein ubiquitination identification team.
- Laboratory facilities and operating procedures that meet international standards.
Service Process
Creative Proteomics has long been committed to the research of ubiquitin-proteomics, can provide high-quality protein ubiquitination identification service for those engaged in proteomics research, and solve any problems encountered in the experiment. If you are interested in this service, please contact us today for technical support.
The service is for research only, not for clinical use.
