Creative Proteomics has been engaged in metabolomics research for a long time, has unique insights into metabolomics, and can provide measurement services for the collision cross-section of biomolecules.
Intermolecular collisions are essentially intermolecular scattering processes under the action of molecular forces. The collision section is an "effective area", which is defined as the area around a particle. For a collision to occur, the center of the other particle must be in this region. The collision cross section is an important distinguishing feature of ions in the gas phase. It is related to its chemical structure and three-dimensional conformation. Ion mobility measures the rotationally averaged collision cross-section of an ion. The measurement of the collisional cross section is of great significance for understanding the role and function of biomolecules in biological systems, and understanding their composition and structure.
Service Content of Measurement of Collisional Cross-Section
At Creative Proteomics, we use ion mobility spectrometry (IMS) and H/D exchange to measure the collisional cross-section and tertiary structure of metabolites. With the continuous improvement of detection technology, we can also use the time-frequency analysis-based ion measurement method in quadrupole ion trap to measure the collision cross-section. To achieve a more accurate analysis, we used high-resolution Fourier-transform ion cyclotron resonance mass spectrometry to measure the collisional cross-sections of biomolecular ions.
Mass Spectrometry Facilities and Projects
- Bruker timsTOF Pro Mass Spectrometer
- Bruker Elute Ultra-High-Performance Liquid Chromatograph
- Hamilton STARlet Automated Liquid Handling System
- Thermo Orbitrap Fusion Tribrid Mass Spectrometer
- Thermo Vanquish Horizon Ultra-High-Performance Liquid Chromatograph
Requirements for Samples
- We focus on endogenous, microbial, and exogenous small molecules (< 2000 Da) in various matrices including cell lysates, tissues, and biological fluids (e.g., plasma, urine).
- Please contact us for specific sample requirements.
- Please provide the specific concentration, volume, preparation time, and source of each sample. And inform the sample information and control and experimental samples (if there are groups, describe the group analysis information in detail.).
Deliverables
- Experimental steps
- Relevant mass spectrometry parameters
- Details of the identified phosphorylation sites
- Mass spectrometry images
- Raw data
Advantages of Measurement of Collisional Cross-Section
- We have extensive and comprehensive mass spectrometry facilities.
- A variety of detection methods are guaranteed to meet your experimental needs.
- Can help you study the unfolding phenomenon of ubiquitin ions with different charge numbers.
Service Process
Creative Proteomics, a leader in the mass spectrometry industry, is constantly updating its facilities and improving its detection capabilities in the hope of providing you with a satisfactory service. If you have any interest in our measurement service of collisional cross-section, please feel free to contact us .
Reference
- Mao, L.; et al. Collision cross section measurements for biomolecules within a high-resolution Fourier transform ion cyclotron resonance cell. Anal Chem. 2015, 87(8): 4072-4075.
The service is for research only, not for clinical use.
