Mass spectrometry-based metabolomics is a comprehensive study of small molecules in cells and tissues using mass spectrometry. The interaction of these small molecules in life activities is called the metabolome. Studying the metabolome can reveal changes in the body's metabolites levels caused by pathological lesions, gene mutations, drug effects, etc. It provides the theoretical basis in many fields such as drug development. Creative Proteomics has the industry's most advanced mass spectrometry facilities and a professional metabolomics analysis team, which can quickly and efficiently provide you with one-stop metabolomics analysis services from sample preprocessing to mass spectrometry data analysis.
The basic principle of metabolomics analysis is to use an electric field to ionize the substance to be measured in an ion source to generate charged ions with the different charge to mass ratios, which are then accelerated into ion beams and finally focused separately using a mass spectrometry facility to obtain mass spectra. Mass spectrometry-based metabolomics analysis quantifies metabolite concentrations and chemical structures of metabolites.
Mass spectrometry-based metabolomics analysis can provide detailed characterization data for accurately analyzing hundreds to thousands of metabolites. Creative Proteomics can provide metabolomics analysis of easy-to-handle samples such as blood, serum, etc., in addition to accepting samples from cells, tissues, organs, or bacteria.
The services we can provide are as follows.
This service enables the analysis of water-soluble metabolites, some lipids, and metabolites of organic acids using GC-MS technology. LC-MS technology can also be used to analyze metabolites such as amino acids, sugars, alcohols, organic acids, and amines. It is our mission to help you explore the overall metabolic characteristics or the laws of the biological activity of living organisms.
We use LC-MS, GC-MS, NMR, and other technologies to unbiasedly detect all small molecule metabolites in cells, tissues, organs, or organisms before and after stimulation or perturbation, reveal their changes, and develop biomarkers for disease, drugs research on the mechanism of treatment and other research provides the basis.
This service primarily uses selective ion monitoring (SIM) on a GC/MS system or targeted MS/MS with multiple reaction ion monitoring (MRM) on a triple quadrupole LC/MS system. It can provide you with more accurate and sensitive targeted analysis data.
We use the UPLC-MS system composed of a mass spectrometer and chromatograph with an electrospray ionization (ESI) source to perform semi-targeted analysis of metabolites and obtain good performance in terms of metabolite coverage, reliability, etc., to quickly promote your research projects.
This service can perform a qualitative analysis of metabolites with higher concentrations, obtain more complete and rich metabolite information, find out the different metabolites in samples, and provide a comprehensive research perspective for metabolomics research. It can help you with marker screening, molecular mechanism research, and multi-omics integration analysis.
We choose specific sample pretreatment methods and optimize the detection conditions of mass spectrometry, which can target bile acids, amino acids, short-chain fatty acids, medium and long-chain fatty acids, nucleotides, reducing sugars, etc. At the same time, we use isotope labeling to ensure high sensitivity, high accuracy, high specificity, and high reproducibility of detection and analysis.
In this service, we select appropriate isotopes for labeling according to different sample characteristics and combine them with LC-MS or MALDI-TOF-IMS analysis to evaluate target metabolites in mixed samples such as plasma, feces, intestinal contents, and tissues.
This service overcomes the problem that classical metabolomics cannot deduce about metabolic turnover (flux), and uses stable isotope labeling to quickly and harmlessly infer metabolic pathways, revealing some potential biomarkers, new metabolic pathways, and treatments target.
We can provide detection data acquired from various mass spectrometry facilities such as LC-MS, high-resolution mass spectrometry (HRMS), GC-MS, nuclear magnetic resonance (NMR), etc. Analyze and compare databases to derive molecular formulas and predicted structures of metabolites.
We help you perform in-depth metabolite studies with analytical methods that measure ion-to-mass ratios to determine the molecular mass of unmodified synthetic biomolecules, and modified synthetic and natural biomolecules.
Our MS/MS or MSn assay service is suitable for qualitative or quantitative analysis of target compounds in animal and plant samples, and can also be used to detect drug metabolism.
We use mass spectrometry techniques such as ion mobility spectrometry (IMS) and H/D exchange to measure the collisional cross section and tertiary structure of metabolites, which can help you reveal the role and function of metabolites in biological systems.
Creative Proteomics, a leader in mass spectrometry-based analysis services, can provide you with comprehensive and customizable metabolomics analysis services. If you are interested, please feel free to contact us .
The service is for research only, not for clinical use.
