Protein-Protein Interactions Identification

Creative Proteomics has extensive experience in proteomic analysis and provides you with high-resolution protein-protein interaction (PPI) identification service with a well-established and advanced technology platform.

Protein-protein interactions are highly specific physical contacts between protein molecules due to interactions such as hydrogen bonding. Proteins can hardly function by themselves in organisms, and many physiological processes rely on protein-protein interactions to function. To identify protein interactions is to identify proteins that have interacting relationships. Using mass spectrometry identification technology, identify the protein that can interact with a specific protein in the experiment, to determine the interacting protein of a specific protein. This is of great significance for revealing the functional structure of proteins, deeply understanding the physiological processes involved in proteins, and discovering protein targets.

Service Content of Protein-Protein Interactions Identification

In the mass spectrometry-based protein interaction identification service provided by Creative Proteomics, co-immunoprecipitation (CO-IP), GST fusion protein Pull-down technology, and SILAC combined with mass spectrometry are usually used for analysis. These methods have their advantages. The co-immunoprecipitation method is mainly to separate and identify the interacting protein complexes in the natural state; the GST fusion protein Pull-down technology can eliminate intracellular interference. In our protein interaction experiments, we first separated the interacting proteins and then used mass spectrometry to identify and analyze the obtained protein of interest/interacting protein. For the identification of known proteins that do not require high accuracy, primary mass spectrometry can complete the identification by obtaining peptide mass fingerprints; if the accuracy is extremely high or for unknown proteins, tandem mass spectrometry can be used for identification.

Mass Spectrometry Facilities and Projects

• Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer
• Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer
• Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole-Orbitrap Mass Spectrometer

Requirements for Samples

• Please confirm that the total protein is greater than 20 µg, and the solution and lyophilized powder are acceptable.
• Please provide the specific concentration, volume, preparation time, and source of each sample. And inform the sample information as well as the control and experimental samples (if there is a group, describe the group information in detail.)

Deliverables

• Experimental steps
• Relevant mass spectrometry parameters
• Details of the identified phosphorylation sites
• Mass spectrometry images
• Raw data

Applications of Protein-Protein Interactions Identification

• Explore the mechanisms of disease development.
• Determination of large-scale protein interaction network mapping.
• Finding new drug targets, developing new drugs, and pioneering new ways to treat diseases.

Service Process

Creative Proteomics is an expert in protein interaction identification, and we can provide an extremely accurate protein-protein interactions identification service. If you are interested in our protein interaction identification service, don't hesitate to contact us today for a quote.