TMT-Based Proteomics Analysis

Creative Proteomics has a unique insight into TMT-based proteomics analysis. With a strong experimental team and mass spectrometry facilities representing international standards, we can provide you with a full-flow TMT-based proteomics analysis service to drive your research forward.

TMT is a technique commonly used to identify and quantify proteins in different samples. The amine-reactive NHS-ester groups, mass normalization groups, and MS/MS reporter groups are the main TMT labeling reagents. The principle of this technology is that the isotope labels of different labeling reagents have the same precursor mass, the peptides after enzymatic hydrolysis are labeled with specific amino acid groups, and finally, the detection reporter group is used. TMT technology can use up to 11 stable isotope labels, can compare the relative content of proteins in up to 10 different samples at the same time, and can label almost all peptide or protein samples, so TMT technology is widely used in the research of protein biomarker discovery.

Service Content of TMT-Based Proteomics Analysis

"Silent" in MS mode, the TMT-based proteomics analysis service quantifies only the reporter ions in the MS/MS spectrum. Creative Proteomics uses mass spectrometry facilities to measure the ionic strength of the fragment reporter group released by MS/MS labeling during peptide fragmentation. In this service, for amine-reactive labels, the normalized region maintains the label at a constant mass of 229. Therefore, using these labels, multiplexing experiments of 2-8 folds can be performed. TMT-based proteomic analysis has the characteristics of high parallelism, high accuracy, and high throughput, and is a high-throughput screening technology commonly used in quantitative proteomics in recent years. We can provide a range of services from sample processing to bioinformatics analysis.

Mass Spectrometry Facilities and Projects

• Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer
• Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer
• Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole-Orbitrap Mass Spectrometer

Requirements for Samples

• Animal tissue samples (including blood/urine/cerebrospinal fluid/lavage fluid/joint fluid), cell samples, microbial (bacteria/fungus) samples, etc. are acceptable.
• Please use sufficient dry ice to transport samples.
• Please provide the specific concentration, volume, preparation time, and source of each sample. And inform the sample information as well as the control and experimental samples (if there is a group, describe the group information in detail.)

Deliverables

• Experimental steps
• Relevant mass spectrometry parameters
• Details of the identified phosphorylation sites
• Mass spectrometry images
• Raw data

Our Advantages

• Perfect laboratory management system to ensure reliable data science.
• More competitive service prices in the industry, with transparent charges.
• Provide complete technical solutions and professional analysis services.
• The experimental technology has the characteristics of high parallelism, high accuracy, and high throughput.

Service Process

The technical team of Creative Proteomics has been engaged in proteomics-related analysis services for a long time and has accumulated rich experimental experience. We can provide you with scientific advice in many fields. If you are interested in our TMT-based proteomic analysis service, please contact us today to discuss the details with an expert.