Creative Proteomics relies on experienced scientists and advanced mass spectrometry facilities to provide you with a customizable, precise quantitative analysis service of sugars to advance your research in food, agriculture, metabolism, and more.
Carbohydrates are an important class of organic compounds that are widely distributed in nature. It plays an important role in the process of life activities, serving as bioenergy sources, carbon sources for the biosynthesis of other substances, structural substances of living organisms, and as a variety of physiologically active functions such as cell recognition and immune activity. As carbohydrates are the main source of energy required by all living organisms to sustain their life activities, the quantitative analysis of sugars is particularly important. With the continuous improvement of mass spectrometry technology, it has been widely applied to the quantitative determination of sugars.
Service Content of Quantitative Analysis of Sugars
Scientists at Creative Proteomics have established several well-established mass spectrometry methods for the quantitative analysis of sugars. We were able to optimize the hydrolysis of polysaccharides by orthogonal design and then use high-performance liquid chromatography/electrospray ionization-ion trap-time-of-flight mass spectrometry (HPLC-ESI-TOF-MS) technology to analyze the monosaccharides in the samples. We can quantify disaccharides by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) using a graphene oxide matrix. We can also combine high-performance liquid chromatography (HPLC-RID) with charged spray liquid chromatography-mass spectrometry (LC-MS) for ionization (ESI) and selected ion monitoring (SIM) of glucose etc. At the same time, we were able to quantitatively determine the total amount of individual disaccharides using liquid chromatography and tandem mass spectrometry (LC-MS/MS).
Mass Spectrometry Facilities and Projects
LC-Triple Quad Mass Spectrometer
Thermo Scientific™ Orbitrap™ Elite Hybrid Ion Trap-Orbitrap Mass Spectrometer
Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole-Orbitrap Mass Spectrometer
Requirements for Samples
- Creative Proteomics can accept a variety of sample types.
- Please provide detailed sample information and potentially multiple biological replicates.
- Please consult our staff for specific requirements for each sample type.
Deliverables
- Experimental steps
- Relevant mass spectrometry parameters
- Details of the identified phosphorylation sites
- Mass spectrometry images
- Raw data
Applications of Quantitative Analysis of Sugars
- Disease infection
- Tumor screening
- Food analysis
- Food development, etc.
Service Process
Scientists at Creative Proteomics have accumulated rich service experience in sugar analysis, and rely on the established mass spectrometry technology platform to provide researchers around the world with customizable quantitative analysis service of sugars. If you have any questions about our quantitative analysis service of sugars, please feel free to contact us.
References
- Guo, N.; et al. Quantitative Analysis of Polysaccharide Composition in Polyporus umbellatus by HPLC-ESI-TOF-MS. Molecules. 2019, 24(14): 2526.
- Bilskey, S. R.; et al. A Comparative Analysis of Methods for Quantitation of Sugars during the Corn-to-Ethanol Fermentation Process. SLAS Technol. 2020, 25(5): 494-504.
- Lee, D.; et al. MALDI-MS analysis of disaccharide isomers using graphene oxide as MALDI matrix. Food Chem. 2021, 342: 128356.
The service is for research only, not for clinical use.
