The sequence of multiple amino acids after dehydration condensation is the protein's primary structure. The primary structure of a protein affects other higher-order structures of the protein and defines the function of the protein. Therefore, to study unknown proteins and proteins in new species, it is first necessary to obtain their amino acid sequence before further research on the structure and function of the protein can be carried out. As a world-class proteomics service provider, Creative Proteomics can provide customers with professional, mass spectrometry-based protein sequencing services to quickly reveal protein sequences and advance your scientific projects.
Creative Proteomics has a complete mass spectrometry technology platform, which can complete the terminal (N-terminal or C-terminal) sequencing of proteins, de novo sequencing, and analysis of mutation sites. Our protein sequencing service starts with SDS-PAGE separation of the protein mixture, followed by LC-MS mass spectrometry analysis after the target is digested. Finally, the peptide sequences determined by primary MS and secondary MS were compared with the database to obtain the exact sequence of the protein.
Mass spectrometry-based protein sequencing can split peptides faster, with more sensitive sequencing results, and can identify end-blocked or modified protein samples. Our protein sequencing service can help you analyze the high-level structure of proteins and reveal the biology of proteins It also plays an important role in drug development. We accept multiple protein samples and provide 24/7 protein sequencing services.
The services we can provide are as follows.
We employ advanced mass spectrometry facilities such as ESI-TOF and MALDI TOF to detect blocked and modified protein C-terminal. By sequencing the C-terminal, it can be judged whether the recombinant protein is fully expressed, whether the expression is fragmented and whether the expression is modified.
We were able to use a combination of two to three different proteases to increase the coverage of the sequence. This service provides detailed data on protein N-terminal and post-translational modifications (PTMs).
For general peptides, we usually use different proteases to digest them separately and then perform de novo sequencing. For short peptide chains, we can use the secondary fragmentation method to align and splice the fragmented peptide fragments before performing de novo sequencing.
Creative Proteomics, a leader in mass spectrometry-based analysis services, can provide you with comprehensive and customizable mass spectrometry-based protein sequencing services. If you are interested, please feel free to contact us .
The service is for research only, not for clinical use.
