Isotopic Labeling Assisted Metabolomics

Creative Proteomics enables the rapid application of isotope labeling methods to metabolomics services. The combined analysis based on stable isotopes and metabolomics allows for the more accurate study of the specific metabolic pathways of a particular metabolite in the metabolic network, effectively advancing your scientific projects.

Isotope labeling-assisted metabolomic analysis is an advanced analytical method to determine not only the total amount of metabolites after they have completed complex metabolism in the body but also to detect the metabolic pathways of metabolites accurately. The principle is to use non-radioactive, physically stable, and harmless stable isotopes to label metabolites, and then infer the metabolic pathways by detecting the traces of isotopic markers through mass spectrometry. This technique can reveal several potential biomarkers, new metabolic pathways, and therapeutic targets, and has played a significant role in metabolic studies of various diseases, especially tumors.

Service Content of Isotopic Labeling Assisted Metabolomics

Our isotopic labeling assisted metabolomics analysis service focuses on the information on dynamical metabolic processes. Stable isotope labeling is used to introduce a dynamic dimension of information analysis based on multiple platforms (CE-MS and LC-MS) to overcome the limitations of classical metabolomics studies where metabolic turnover rates (throughput) cannot be derived. We use 13C to label substrates and can analyze intracellular metabolites produced by substrate metabolism as well as to determine metabolic secretion and uptake rates. Combining the assay data with known biochemical response networks enables the calculation of metabolic fluxes and even the prediction of network response after the introduction of modifications.

Mass Spectrometry Facilities and Projects

• Thermo Scientific™ UHPLC-Q Exactive Orbitrap Mass Spectrometer
• Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer
• Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer

Requirements for Samples

• Microbial and cellular samples: rapidly inactivates metabolic activity (quenching) while keeping cells from lysing.
• Animal body fluids: pre-treatment should be performed quickly after sampling, such as adding anticoagulants and preservatives, and immediately freezing (-80℃).
• Plant samples: frozen (liquid nitrogen) immediately after collection, and then transferred to -80℃ for storage, 200 mg/case.
• Serum sample: 150 µL/case, must avoid repeated freezing and thawing. Blood was collected in centrifuge tubes and allowed to stand for 30 minutes for coagulation. Centrifuge at 8000 rpm for 10 minutes, take the supernatant and aliquot into cryopreservation tubes and store in liquid nitrogen or -80℃ for cryopreservation.
• For other sample collection methods, please consult our staff.

Deliverables

• Experimental steps
• Relevant mass spectrometry parameters
• Details of the identified phosphorylation sites
• Mass spectrometry images
• Raw data

Applications of Isotopic Labeling Assisted Metabolomics

• It can be used to discover abnormal metabolic characteristics and further functional mechanisms.
• It can be used to study qualitative changes in pathway contributions as well as substrate contributions to metabolite pools and integration issues.
• It can be used for multi-dimensional metabolomic analysis, and simultaneously obtain static metabolite accumulation content and dynamic metabolic process change information.

Service Process

Creative Proteomics specializes in metabolomics-related research, and our team can provide professional isotope labeling assisted metabolomics analysis service. If you are interested in this service, please contact us today for more information and a quote.