Creative Proteomics is an expert in the analysis of N-terminal sequences. Based on sophisticated and advanced mass spectrometry facilities, we are able to provide customers with personalized N-terminal sequence analysis solutions. We are confident to meet all your needs to advance your research.
The N-terminus is the starting point of a protein or polypeptide, and the sequence composition of the N-terminus of a protein has a huge impact on the overall biological function of the protein. It affects the subcellular distribution, degradation, and transformation rates of proteins. The analysis of the N-terminal sequence of the protein is beneficial to help analyze the high-level structure of the protein, reveal the biological function of the protein, and also has important significance for drug development. At present, there are two main methods for protein N-terminal sequencing, one is non-mass spectrometry and the other is mass spectrometry. Creative Proteomics mainly provides customers with N-terminal sequence analysis services based on mass spectrometry facilities.
Service Content of N-Terminal Sequence Analysis
In the N-terminal sequence analysis service, we can provide high-throughput N-terminal sequencing services through high-resolution mass spectrometry instruments. First, we can increase sequence coverage by using a combination of two or three different proteases. Combined with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) to sequence the N-termini of these sequences, detailed data on N-terminal blocking and post-translational modifications (PTMs) can be obtained.
Mass Spectrometry Facilities and Projects
- ESI-TOF Mass Spectrometer
- Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS)
Requirements for Samples
- Protein samples can be dry powders, solutions, or fixed on PVDF membranes and stained with Ponceau.
- Sample concentration and purity: concentration > 50 pmol, purity > 90% (recommended sample volume is more than 5 times the required amount).
- Salt content: non-volatile inorganic salts < 5 mM, volatile inorganic salts < 20 mM, Tris buffer system cannot be used.
- Please provide the specific concentration, volume, preparation time, and source of each sample. And inform the sample information as well as the control and experimental samples (if there is a group, describe the group information in detail.)
Deliverables
- Experimental steps
- Relevant mass spectrometry parameters
- Details of the identified phosphorylation sites
- Mass spectrometry images
- Raw data
Applications of N-terminal Sequence Analysis
- Probe the higher structure and physiological function of proteins.
- Demonstrate the consistency between batches of proteins or peptides.
- Demonstrate that the N-terminal end of a protein is intact and meets expectations in drug development.
Service Process
As a professional protein sequence analysis company, Creative Proteomics looks forward to providing you with a one-stop N-terminal sequence analysis service. If you are interested in our N-terminal sequence analysis service, please contact us directly for details and a quote.
The service is for research only, not for clinical use.
