Inhibitor Binding Site Determination

Creative Proteomics has rich experience in the identification of inhibitor binding sites. With a professional team and advanced mass spectrometry facilities, we can provide you with a one-stop inhibitor binding site identification service from experimental design to result in delivery, speeding up your Research progress.

Protein inhibitors include protease inhibitors, protein synthesis inhibitors, and the like. Playing an important role in cell activities, protease inhibitors are a kind of chemical similar to drugs for the treatment of AIDS; protein synthesis inhibitors are usually involved in inhibiting all aspects of protein synthesis in bacterial cells. Using mass spectrometry to identify various inhibitor binding sites can more clearly define the region where the inhibitor selectively binds to the protein and the structural characteristics of the region, providing important ideas for drug development.

Service Content of Inhibitor Binding Site Determination

In inhibitor binding site identification service, Creative Proteomics utilizes specialized mass spectrometry facilities (MALDI-MS or ESI-MS) to identify inhibitor binding sites. While there are many uncertainties in the identification of inhibitor binding sites, our extensive experience guarantees customer satisfaction. Based on the structural properties of inhibitor binding sites, we can optimize the design of personalized protocols and even identify protein inhibitor binding sites at the atomic level.

Mass Spectrometry Facilities and Projects

• Electrospray Ionization Mass Spectrometry (ESI-MS)
• Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer
• Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer
• Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS)
• Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole-Orbitrap Mass Spectrometer

Requirements for Samples

• Solution (target protein): total target protein > 50 μg, target protein concentration > 80%.
• Solution (large-scale mixed protein): total protein > 1 mg, protein concentration > 1 μg/μL.
• Please note that for plasma samples use EDTA solution to prevent coagulation. And store all samples at -80°C, do not freeze and thaw samples repeatedly, and use sufficient dry ice for transportation.
• Please provide the specific concentration, volume, preparation time, and source of each sample. And inform the sample information as well as the control and experimental samples (if there is a group, describe the group information in detail.)

Deliverables

• Experimental steps
• Relevant mass spectrometry parameters
• Details of the identified phosphorylation sites
• Mass spectrometry images
• Raw data

Our Advantages

• Fast experimentation cycles to move your projects forward.
• Rich experimental experience can solve any problems in your project.
• Strict laboratory management system, the experimental data is true and reliable.
• Various types of samples are accepted, including tissue samples, cell samples, etc.

Service Process

Creative Proteomics has internationally recognized professional mass spectrometry facilities and a technical team that has long been engaged in inhibitor binding site identification services, which can accurately solve your problems in inhibitor binding site research and meet all your needs. If you are interested in our inhibitor binding site identification service, please contact us today for technical support.

References

1. Davis, M. F.; et al. Determination of separate inhibitor and substrate binding sites in the dehaloperoxidase-hemoglobin from Amphitrite ornata. Biochemistry. 2010, 49(6): 1199-1206.
2. Shkriabai, N.; et al. Identification of an inhibitor-binding site to HIV-1 integrase with affinity acetylation and mass spectrometry. Proc Natl Acad Sci U S A. 2004, 101(18): 6894-6899.