Protein and Peptide Molecular Weight Determination

Creative Proteomics has been engaged in protein-related detection services for a long time. With our high-quality mass spectrometry platform and professional identification team, we can provide you with a high-quality protein and peptide molecular weight determination service.

One of the most basic and important aspects of protein research is the determination of protein molecular weight. Molecular weight is the key to further study of protein physiological structure. Molecular weight, a basic physical and chemical property parameter have important reference significance for amino acid sequence determination, unknown structure determination, drug development, and drug metabolism analysis. With the increasing maturity of biological mass spectrometry, more and more new technologies have been applied to protein research. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is widely used in protein and peptide molecular weight determination.

Service Content of Protein and Peptide Molecular Weight Determination

Creative Proteomics offers a variety of assay protocols for protein and peptide molecular weight determination in this service. Based on an advanced MALDI-TOF mass spectrometer, our experimental accuracy is far ahead of the industry. Our protein assay services can measure a wide range of molecular weights of biological macromolecules and meet almost every protein assay requirement on the market. We not only provide protein and peptide molecular weight determination service for the majority of scientific researchers but also provide downstream experiments that can further analyze sample purity and detect complex protein structures.

Mass Spectrometry Facilities and Projects

• Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole-Orbitrap Mass Spectrometer
• Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS)

Requirements for Samples

• The required amount of the protein sample is about 10-50 µg, and the sample purity is > 90%;
• Try to avoid the use of surfactants, such as SDS, Triton-X, etc., during sample preparation. Salt concentrations, such as Tris, are also minimized. Please store all samples at -80°C and do not freeze and thaw samples repeatedly.
• Please provide the specific concentration, volume, preparation time, and source of each sample. And inform the sample information as well as the control and experimental samples (if there is a group, describe the group information in detail.)

Deliverables

• Experimental steps
• Relevant mass spectrometry parameters
• Details of the identified phosphorylation sites
• Mass spectrometry images
• Raw data

Our Advantages

• Easy, intuitive determination of macromolecular mixtures with essentially no fragmentation peaks.
• Strict experimental regulations and a professional technical team can meet your experimental needs to the maximum extent.
• It has the advantages of low sample consumption, high sensitivity, fast determination speed, high throughput, and large-scale identification.

Service Process

Creative Proteomics have a professional technical service team, which can provide you with customizable, scientifically feasible experimental plans to quickly advance your research projects. If you are interested in our protein molecular and peptide weight determination service, please contact us today for a solution.