Creative Proteomics has extensive experience in protein post-translational modification identification. With our professional team and advanced mass spectrometry facilities, we can provide you with a one-stop protein proline hydroxylation identification service from experiment design to result in delivery to accelerate your research progress.
Protein proline hydroxylation, which is very common in plants and animals, is a process in which oxygen is added to the pyrrolidine ring of proline by a conserved prolyl hydroxylase, eventually forming a hydroxyl group in the (S) configuration on the γ-carbon atom of proline. Proline hydroxylated proteins are involved in various physiological activities such as animal cell proliferation and plant growth and development. Especially in animal cells, it is involved in tumor cell proliferation and angiogenesis processes, etc. It is a critical oxygen-sensitive post-translational modification. However, studies on protein proline hydroxylation are still limited, and the completion of protein proline hydroxylation identification can provide strong data support to study its functional characteristics.
Service Content of Protein Proline Hydroxylation Identification
In the protein proline hydroxylation identification service, the professional technical team of Creative Proteomics will provide you with a scientifically feasible and customized experimental plan to meet all your project needs. We identified proline hydroxylation of proteins by LC-MS/MS from Thermo Scientific. The identification of hydroxyproline-containing peptide substrates can also be accomplished by HPLC/MS analysis of enriched peptide samples with or without immunoaffinity. All of our data will be compared and identified in the appropriate protein database, and the original data and corresponding comparison reports will be provided.
Mass Spectrometry Facilities and Projects
- Triple Quadrupole LC-MS/MS (ABI Sciex API 4000 and/or 4500)
- Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer
- High Performance Liquid Chromatography Mass Spectrometry (HPLC/MS)
- Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer
- Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole-Orbitrap Mass Spectrometer
Requirements for Samples
- Solution (target protein): total target protein > 50 μg, target protein concentration > 80%.
- Solution (large-scale mixed protein): total protein > 1 mg, protein concentration > 1 μg/μL.
- Please store all samples at -80°C, do not freeze and thaw samples repeatedly, and use sufficient dry ice for transportation.
- Please provide the specific concentration, volume, preparation time, and source of each sample. And inform the sample information as well as the control and experimental samples (if there is a group, describe the group information in detail.)
Deliverables
- Experimental steps
- Relevant mass spectrometry parameters
- Details of the identified phosphorylation sites
- Mass spectrometry images
- Raw data
Our Advantages
- Competitive service prices and quality service offerings.
- Provides critical data support for cancer treatment research.
- State-of-the-art laboratory facilities and experienced laboratory team.
Service Process
Creative Proteomics has long been engaged in protein profiling services and has a good reputation in the industry. We guarantee to provide you with high-quality and convenient protein proline hydroxylation identification service to ensure that the problems you encounter in your project are solved. If you are interested in our protein proline hydroxylation identification service, please contact us today for technical support.
References
- Liang, R.; et al. Identification of Hydroxyproline-Containing Proteins and Hydroxylation of Proline Residues in Rice. Front Plant Sci. 2020, 11: 1207.
- Zhou, T.; et al. Proteomic analysis reveals diverse proline hydroxylation-mediated oxygen-sensing cellular pathways in cancer cells. Oncotarget. 2016, 7(48): 79154-79169.
The service is for research only, not for clinical use.
